1. 新型医学光学成像技术开发及其在疾病诊断、疗效监测中的应用

2. 光动力疗法剂量监测技术及临床应用

个人主页：https://www.researchgate.net/profile/Defu-Chen-3

https://orcid.org/0000-0002-7198-1104

1. 《生物医学光子学与应用》（秋季学期），32学时

1. 国家自然科学基金委青年项目，62205025，宫颈管癌变的环扫荧光内窥光敏剂定量方法研究，2023-01至2025-12，30万，在研，主持

2. 国家重大科研仪器研制项目，62227823，血管靶向光动力剂量参数定量监测仪，2023-01至2027-12，80 万，在研，合作单位主持

3. 北京市自然科学基金面上项目，7222309，光动力治疗宫颈癌前病变的光敏剂空间分布定量监测方法研究，2022-01至2024-12，20万，在研，主持

4. 武汉光电国家研究中心开放基金课题，2020WNLOKF025，宫颈癌前病变的光动力精准治疗技术研究，2021-01至2023-12，12万，在研，主持

5. 北京理工大学青年教师学术启动计划，XSQD-202123001，光动力治疗宫颈癌前病变的剂量监测技术研究，2020-07至2023-07，40万，在研，主持

6. 博士后国际交流计划派出项目，无创超高分辨光学相干层析成像技术监测血管靶向光动力效应，2016-10至2019-10，30万，结题，主持

7. 国家自然科学基金委，重点项目，61835015，调控血管靶向光动力治疗实时在体光学相干层析成像造影关键技术研究，2019-01至2023-12，278万，在研，参与

8. 国家自然科学基金委，重点项目，61635014，增强血管靶向光动力效应的时空规律及调控研究，2017-01至2021-12，280万，结题，参与

9. 国家重点研发计划，LED用于健康与医疗的机理、方法、设备与应用研究，2017-01至2022-06，结题，参与
10. 国家自然科学基金委，重大科研仪器研制项目，61527827，可视化光动力治疗肿瘤的电子内窥镜系统，2016-01至2021-12，535万，结题，参与

11. 国家自然科学基金委，面上项目，61675236，血管靶向光动力中激光光热效应规律的实验及数学模型研究，2017-01至2020-12，66万，结题，参与

12. 国家自然科学基金委，应急管理项目，61450005，电子胃镜下光动力治疗肿瘤的可视化技术研究，2015-01至2015-12，40万，结题，参与

[1] D. F. Chen, D. W. Nauen, H. C. Park, D. W. Li, W. Yuan, A. Li, H. H. Guan, C. Kut, K. L. Chaichana, C. Bettegowda, A. Quiñones-Hinojosa, X. D. Li. Label-free imaging of human brain tissue at subcellular resolution for potential rapid intra-operative assessment of glioma surgery. Theranostics, 2021, 11(15): 7222-7234. (Back cover)

[2] D. F. Chen, Y. Wang, H. Y. Zhao, H. X. Qiu, Y. T. Wang, J. Yang, Y. Gu. Monitoring perfusion and oxygen saturation in port-wine stains during vascular targeted photodynamic therapy. Annals of Translational Medicine, 2021, 9(3): 214.

[3] D. F. Chen, W. Yuan, H. C. Park, X. D. Li. In vivo assessment of vascular-targeted photodynamic therapy effects on tumor microvasculature using ultrahigh-resolution functional optical coherence tomography. Biomedical Optics Express, 2020, 11(8): 4316-4325.

[4] W. Yuan, D. F. Chen, R. Sarabia-Estrada, H. Guerrero-Cázares, D. W. Li, A. Quiñones-Hinojosa, X. D. Li. Theranostic OCT microneedle for fast ultrahigh-resolution deep-brain imaging and efficient laser ablation in vivo. Science Advances, 2020, 6: eaaz9664.

[5] D. F. Chen, J. Ren, H. Y. Lin, B. H. Li, Y. Gu. Intraoperative monitoring blood perfusion of port wine stains by laser Doppler imaging during vascular targeted photodynamic therapy: A preliminary study. Photodiagnosis and Photodynamic Therapy, 2016, 14: 142-151.

[6] D. F. Chen, J. Ren, Y. Wang, H. Y. Zhao, B. H. Li, Y. Gu. Relationship between the blood perfusion values determined by laser speckle imaging and laser Doppler imaging in normal skin and port wine stains. Photodiagnosis and Photodynamic Therapy, 2016, 13:1-9.

[7] D. F. Chen, Y. Wang, H. Y. Lin, B. H. Li, X. C. Lin, and Y. Gu. Effects of pulse width and repetition rate of pulsed laser on kinetics and production of singlet oxygen luminescence. Journal of Innovative Optical Health Sciences, 2016, 9(5): 1650019.

[8] D. F. Chen, Y. Wang, B. H. Li, Y. Gu. Influence of pulse repetition rate on singlet oxygen production during photosensitization using a pulsed laser. Photonics & Lasers in Medicine, 2015, 4(4): 312-314.

[9] D. F. Chen, H. F. Zheng, H. Y. Lin, H. Y. Lin, Z. D. Ke, S. S. Xie, B. H. Li. Light-emitting diode based illumination system for in vitro photodynamic therapy. International Journal of Photoenergy, 2012, 2012: 920671.

[10] H. Y. Lin#, D. F. Chen# (Equal Contribution), M. Wang, J. Q. Lin, B. H. Li, S. S. Xie. Influence of pulse-height discrimination threshold for photon counting on the accuracy of singlet oxygen luminescence measurement. Journal of Optics, 2011, 13(12): 125301.

[11] T. Q. Huang#, D. F. Chen# (Equal Contribution), W. D. Su, H. Y. Lin, B. H. Li, S. S. Xie. A new time-gated synchronization controller for the detection of singlet oxygen luminescence. Optoelectronics and Advanced Materials-Rapid Communications, 2010, 4(8):1083-1087.

[12] H. H. Guan, W. X. Liang, A. Li, Y. A. Gau, D. F. Chen, M. J. Li, D. E. Bergles, X. D. Li. Multicolor fiber-optic two-photon endomicroscopy for brain imaging. Optics Letters, 2021, 46(5): 1093-1096.

[13] X. J. Fan, D. F. Chen, J. Zeng, X. Y. Liang, Y. X. Xu, H. X. Qiu, Y. Gu. Impact of Spectral Component of LED Lighting System on Glucose and Lipid Metabolism. Spectroscopy and Spectral Analysis, 2021, 41 (05): 1644-1651.

[14] X. J. Fan, D. F. Chen, Y. Wang, Y. Z. Tan, H. Y. Zhao, J. Zeng, Y. Q. Li, X. H. Guo, H. X. Qiu, and Y. Gu. Light intensity alters the effects of light-induced circadian disruption on glucose and lipid metabolism in mice. AJP-Endocrinology and Metabolism, 2022, 322 (1), E1-E9

[15] Y. D. Liu, D. F. Chen, J. J. Xu, Y. Z. Tan, Y. Wang, H. Y. Zhao, H. Li, H. L. Liu, Y. Gu, H. X. Qiu. Quantitative assessment of vascular features in port wine stains through optical coherence tomography angiography. Photodiagnosis and Photodynamic Therapy. 2021, 36, 102607

[16] Y. Z. Tan, S. K. Sun, D. F. Chen, H. X. Qiu, J. Zeng, Y. Wang, H. Y. Zhao, Y. Gu. Light Delivery Device Modelling for Homogenous Irradiation Distribution in Photodynamic Therapy of Non-spherical Hollow Organs. Photodiagnosis and Photodynamic Therapy. 2021: 102320.

[17] A. Li, H. H. Guan, H. P., Y. L. Yue, D. F. Chen, W. X. Liang, M. J. Li, H. Lu, X. D. Li. Twist-free ultralight two-photon fiberscope enabling neuroimaging on freely rotating/walking mice. Optica, 2021, 8(6): 870-879.

[18] X. W. Yao, D. W. Li, H. C. Park, D. F. Chen, H. H. Guan, M. Mahendroo, X. D. Li. "Ultra-sensitive optical coherence elastography using high-dynamic-range force loading scheme for cervical rigidity assessment," Biomedical Optics Express, 2020, 11(2): 688-698.

[19] W. X. Liang, H. C. Park, K. Y. Li, A. Li, D. F. Chen, H. H. Guan, Y. Yue, Y. A. Gau, D. Bergles, M. J. Li, H. Lu, X. D. Li. Throughput-speed product augmentation for scanning fiber-optic two-photon endomicroscopy. IEEE Transactions on Medical Imaging, 2020, 39(12): 3779-3787.

[20] L. S. Lin, H. Y. Lin, Y. Shen, D. F. Chen, Y. Gu, B. C. Wilson, B. H. Li. Singlet oxygen luminescence image in blood vessels during vascular targeted photodynamic therapy. Photochemistry and Photobiology, 2020, 96: 646-651.

[21] Y. D. Liu, D. Zhu, J. J. Xu, Y. Wang, W. Feng, D. F. Chen, H. L. Liu, X. H. Guo, Y. Q. Li, H. X. Qiu, Y. Gu. Penetration-enhanced optical coherence tomography angiography with optical clearing agent for clinical evaluation of human skin. Photodiagnosis and Photodynamic Therapy, 2020, 30: 101734.

[22] Y. D. Liu, Q. Ang, H. Wu, J. J. Xu, D. F. Chen, H. Y. Zhao, H. L. Liu, X. H. Guo, H. X. Qiu, Y. Gu. Prevalence of human papillomavirus genotypes and precancerous cervical lesions in a screening population in Beijing, China: Analysis of results from China’s Top 3 Hospital, 2009–2019. Virology Journal, 2020, 17:104.

[23] D. W. Li, J. M. Wu, Y. F. He, X. W. Yao, W. Yuan, D. F. Chen, H. C. Park, S. Y. Yu, J. L. Prince, X. D. Li. Parallel deep neural networks for endoscopic OCT image segmentation. Biomedical Optics Express, 2019, 10: 1126-1135.

[24] A. Li, G. Hall, D. F. Chen, W. X. Liang, B. Ning, H. H. Guan, X. D. Li. A biopsy-needle compatible varifocal multiphoton rigid probe for depth-resolved optical biopsy. Journal of Biophotonics, 2018, 12: e201800229.

[25] H. Y. Zhao, R. Yin, Y. Wang, Y. H. Leed, T. Luo, J. Y. Zhang, H. X. Qiu, S. Ambrose, L. J. Wang, J. Ren, J. Yao, D. F. Chen, Y. C. Wang, Z. P. Liang, J. Zhen, S. M. Wu, Z. L. Ye, J. Zeng, N. Y. Huang, Y. Gu. Modulating mitochondrial morphology enhances antitumor effect of 5-ALA-mediated photodynamic therapy both in vitro and in vivo. Journal of Photochemistry & Photobiology, B: Biology, 2017, 176: 81-91.

[26] Z. Qiu, G. Yao, D. F. Chen, Y. Wang, Y. Gu, B. Li. Determination of optical and microvascular parameters of port wine stains using diffuse reflectance spectroscopy. Advances in Experimental Medicine and Biology, 2016, pp 359-365.

[27] Q. L. Zou, H. Y. Zhao, Y. X. Zhao, Y. Y. Fang, D. F. Chen, J. Ren, X. P. Wang, Y. Wang, Y. Gu, F. P. Wu. Effective two-photon excited photodynamic therapy of xenograft tumors sensitized by water-soluble Bis(arylidene)cycloalkanone photosensitizers. Journal of Medicinal Chemistry, 2015, 58: 7949-7958.

[28] J. Ren, P. C. Li, H. Y. Zhao, D. F. Chen, J. Zhen, Y. Wang, Y. C. Wang, Y. Gu. Assessment of tissue perfusion changes in port wine stains after vascular targeted photodynamic therapy: a short-term follow-up study. Lasers in Medical Science, 2014, 29(2):781-788.

[29] Z. H. Qiu, D. F. Chen, Y. Wang, G. P. Yao, Y. Gu, B. H. Li. Monitoring blood volume fraction and oxygen saturation in port-wine stains during vascular targeted photodynamic therapy with diffuse reflectance spectroscopy: results of a preliminary case study. Photonics & Lasers in Medicine, 2014, 3(3): 273-280.

[30] H. Y. Zhao, R. Yin, D. F. Chen, J. Ren, Y. C. Wang, H. Deng, Y. Wang, H. X. Qiu, N. Y. Huang, Q. L. Zou, J. Q. Zhao, Y. Gu. In vitro and in vivo antitumor activity of a novel hypocrellin B derivative for photodynamic therapy. Photodiagnosis and Photodynamic Therapy, 2014, 11: 204-212.

[31] L. S. Lin, H. Y. Lin, D. F. Chen, S. S. Xie, Y. Gu, B. C. Wilson, B. H. Li. Imaging singlet oxygen luminescence in blood vessels. 2014, SPIE Newsroom, DOI: 10.1117/2.1201406.005511.

[32] B. H. Li, H. Y. Lin, D. F. Chen, B. C. Wilson, Y. Gu. Singlet oxygen detection during photosensitization. Journal of Innovative Optical Health Sciences, 2013, 6(1): 1330002.

[33] H. Y. Lin, Yi Shen, D. F. Chen, L. S. Lin, B. C. Wilson, B. H. Li, S. S. Xie. Feasibility study on quantitative measurements of singlet oxygen generation using singlet oxygen sensor green. Journal of Fluorescence, 2012, 23(1): 41-7.

[34] Y. Shen, H. Y. Lin, Z. F. Huang, D. F. Chen, B. H. Li, S. S. Xie. Indirect imaging of singlet oxygen generation from a single cell. Laser Physics Letters, 2011, 8(3): 232-238.

[35] B. H. Li, H. Y. Lin, D. F. Chen, M. Wang, S. S. Xie. Detection system for singlet oxygen luminescence in photodynamic therapy. Chinese Optics Letters, 2010, 8(1): 86-88.

[36] D. F. Chen, D. W. Nauen, X. D. Li, Y. Gu. Optical histology for cancer detection and PDT treatment assessment. Proc. of SPIE, 2020, 11553: 1155318.

[37] D. F. Chen, D. W. Nauen, X. D. Li. Label-free histopathology of human brain tissue with multiphoton imaging. Journal of Neuropathology and Experimental Neurology, 2019, 78 (6), 552-552.

[38] D. F. Chen, J. Ren, Y. Wang, Y. Gu. Laser Doppler line scanner for monitoring skin perfusion changes of port wine stains during vascular-targeted photodynamic therapy. Proc. of SPIE, 9268: 92681Y-1.

[39] D. F. Chen, H. Y. Lin, Yi Shen, B. H. Li, S. S. Xie. Optimal discrimination threshold for the detection of singlet oxygen luminescence. Journal of Physics: Conference Series, 2011, 277: 012004.

[40] H. Lin, Y. Shen, D. F. Chen, L. S. Lin, B. Li, S. Xie. Determination of singlet oxygen quantum yield of HiPorfin using Singlet Oxygen Sensor Green. Proc. of SPIE, 2010, 7845: 78451J.

[41] L. Lin, H. H. Lin, D. F. Chen, L. Chen, M. Wang, S. Xie, Y. Gu, B. C. Wilson, B. H. Li. Direct imaging of singlet oxygen luminescence generated in blood vessels during photodynamic therapy. Proc. of SPIE, 2014, 9129: 912920-1.

[42] J. Zhen, C. Wang, Y. Wang, D. F. Chen, Y. Gu. Optical coherence tomography: A potential tool for prediction of treatment response for port wine stain after photodynamic therapy. Proc. of SPIE, 9268: 926807.

[43] Y. Shen, H. H. Lin, Z. Huang, L. Xiao, D. F. Chen, B. H. Li, S. Xie. Kinetic analysis of singlet oxygen generation in a living cell using Singlet Oxygen Sensor Green. Proc. of SPIE, 2010, 7845: 78451F.

[44] L. Lin, Y. Li, J. Zhang, Z. Tan, D. F. Chen, S. Xie, Y. Gu, B. H. Li. Vessel constriction correlated with local singlet oxygen generation during vascular targeted photodynamic therapy. Proc. of SPIE, 9268: 92680T.

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