用于生物医学的微电子机械系统（Biomedical Micro-Electro-Mechanical System），类器官芯片，可穿戴器件

本科生课程：主讲《生物医学检验仪器》
研究生课程：参与《生物医学工程前沿》

1.Zewen Wei, Zhihong Li, “Methods in Molecular Biology: Electroporation Protocols”, Chapter 8, 2014, New York, Humana Press, Springer.

主持项目
1.国防科技应用推进计划重点项目课题，用于XXXX的可穿戴微针阵列芯片研制，145BKJ050XXXXXX1，2023-2025，380万元，在研，主持。
2.国家重点研发计划课题，病原菌分离富集技术研发与微流控芯片研制，2022YFC2403301，2022至2025，184万元，在研，主持。
3.国家自然科学基金委面上项目，用于肺癌伴随诊断的循环肿瘤细胞单细胞多维度分析芯片，62074016，2021至2024，63万元，在研，主持。
4.国家自然科学基金委员青年科学基金项目，利用柔性衬底微针尖电极阵列实现活体组织基因治疗基础研究，61204118，2013至2015，24万元，结题，主持。
5.首都卫生发展专项课题，2021-1G-1191-2，新冠病毒微流控检测芯片研究，2021至2022，15万元，结题，主持。
6.北京市科学技术研究院委托科研项目，新冠病毒核酸检测微流控芯片研制，2022-2023，40万元，在研，主持。
7.山东省济南市创新创业人才专项，胎儿有核红细胞自动精准提取系统，2022至2024，200万元，在研，主持。
8. 企业委托科技合作项目，肺癌类器官芯片研制及临床应用，2021至2026,300万元，在研，主持。
9.企业委托科技合作项目，肺癌类器官芯片关键技术开发，2021至2023,300万元，在研，主持。
10.北京理工大学原创基础前沿交叉学科创新专项，基于微流控芯片的自动化类器官分析仪研制，2023-2024, 30万元，在研，主持。
11.北京理工大学青年教师学术启动计划，面向乳腺癌精准诊疗的循环肿瘤细胞单细胞分析芯片研究，2019-2022，60万元，结题，主持。

部分论文 *第一作者 #通讯作者
1.Zhang K, Wang Y, Xue J, Liang N#, Wei Z#. Real-time monitoring ATP variation in human cancer organoids for a long term by DNA-based nanosensor. Anal Chim Acta. 2023, 22;1275:341608. 中科院1区Top期刊
2.Zhang K, Huang Z, Wang Y, Xue J, Liang N#, Wei Z#.Rapid determination of the presence of EGFR mutations with DNA-based nanocalipers. Nanoscale, 2023,15, 13834-13841. 中科院2区Top期刊
3.Zhao H, Wang X, Geng Z, Liang N, Li Q, Hu X#, Wei Z#. Dual-function microneedle array for efficient photodynamic therapy with transdermal co-delivered light and photosensitizers. Lab Chip. 2022 Nov 22;22(23):4521-4530. 中科院1区Top期刊
4.Zhang W, Li Q, Jia F, Hu Z, Wei Z#. A Microfluidic Chip for Screening and Sequencing of Monoclonal Antibody at a Single-Cell Level. Anal Chem. 2021 Jul 27;93(29):10099-10105. 中科院1区Top期刊
5.Zhang W, Li R, Jia F, Hu Z#, Li Q#, Wei Z# A microfluidic chip for screening high-producing hybridomas at single cell level. Lab Chip. 2020 Nov 7;20(21):4043-4051. 中科院1区Top期刊
6.Li R, Jia F, Zhang W, Shi F, Fang Z, Zhao H, Hu Z#, Wei Z#. Device for whole genome sequencing single circulating tumor cells from whole blood. Lab Chip. 2019 Sep 27;19(19):3168-3178. 中科院1区Top期刊
7.Li R, Ma Y, Zhao M, Hu Z#, Wei Z#. Device to Study the Cell Invasion Behavior and Phenotypic Profile at Single Cell Level. Anal Chem. 2018 Feb 6;90(3):1691-1700. 中科院1区Top期刊
8.Li R, Zhou M, Yue C, Zhang W, Ma Y, Peng H, Hu Z#, Wei Z#. Multiple single cell screening and DNA MDA amplification chip for oncogenic mutation profiling. Lab Chip. 2018 Feb 27;18(5):723-734. 中科院1区Top期刊
9.Zhao D, Wu M, Huang D, Liang Z#, Wei Z#, Li Z. Parametric optimization of electric field strength for cancer electrochemotherapy on a chip-based model. Theranostics. 2018 Jan 1;8(2):358-368. 中科院1区Top期刊
10. Li R, Zhou M, Li J, Wang Z, Zhang W, Yue C, Ma Y, Peng H, Wei Z#, Hu Z#. Identifying EGFR-Expressed Cells and Detecting EGFR Multi-Mutations at Single-Cell Level by Microfluidic Chip. Nano-Micro Lett. 2018;10(1):16. 中科院1区Top期刊
11.Zhao M, Zhao D, Ma Y, Hu Z, Wei Z#. Quantitative Proteomic Analysis of Cell Responses to Electroporation, a Classical Gene Delivery Approach. Proteomics. 2018 Aug;18(16):e1800127.
12.Zhang W, Li Z, Wang Z, Yue C, Zheng H, Li R, Zhou M, Hu Z#, Wei Z#, Li Q. Generation of a monoclonal antibody recognizing the heavily glycosylated CD45 protein and its application on identifying circulating tumor cells. PLoS One. 2018 Feb 9;13(2):e0192506.
13.Zhou M, Zheng H, Wang Z, Li R, Liu X, Zhang W, Wang Z, Li H, Wei Z#, Hu Z#. Precisely Enumerating Circulating Tumor Cells Utilizing a Multi-Functional Microfluidic Chip and Unique Image Interpretation Algorithm. Theranostics. 2017 Oct 17;7(19):4710-4721. 中科院1区Top期刊
14.Zhao D, Huang D, Li Y, Wu M, Zhong W, Cheng Q, Wang X, Wu Y, Zhou X, Wei Z*, Li Z#, Liang Z#. A Flow-Through Cell Electroporation Device for Rapidly and Efficiently Transfecting Massive Amounts of Cells in vitro and ex vivo. Sci Rep. 2016 Jan 5;6:18469.
15.Ye M*, Wei Z*, Hu F, Wang J, Ge G, Hu Z, Shao M, Lee ST, Liu J. Fast assembling microarrays of superparamagnetic Fe3O4@Au nanoparticle clusters as reproducible substrates for surface-enhanced Raman scattering. Nanoscale. 2015 Aug 28;7(32):13427-37. 中科院2区Top期刊
16.Wei Z*, Huang Y*, Zhao D, Hu Z, Li Z, Liang Z. A pliable electroporation patch (ep-Patch) for efficient delivery of nucleic acid molecules into animal tissues with irregular surface shapes. Sci Rep. 2015 Jan 5;5:7618.
17.Wang W*, Zhang D*, Wei Z*, Wang Z, Bu X, Yang S, Fang Q, Hu Z. Label-free detection microarray for novel peptide ligands screening base on MS-SPRi combination. Talanta. 2015 Mar;134:705-711. 中科院1区Top期刊
18. Wei Z*, Zheng S*, Wang R, Bu X, Ma H, Wu Y, Zhu L, Hu Z, Liang Z, Li Z. A flexible microneedle array as low-voltage electroporation electrodes for in vivo DNA and siRNA delivery. Lab Chip. 2014 Oct 21;14(20):4093-102. 中科院1区Top期刊
19.Wei Z*, Li X, Zhao D, Yan H, Hu Z, Liang Z, Li Z. Flow-through cell electroporation microchip integrating dielectrophoretic viable cell sorting. Anal Chem. 2014 Oct 21;86(20):10215-22. 中科院1区Top期刊
20.Wang W*, Wei Z*, Zhang D, Ma H, Wang Z, Bu X, Li M, Geng L, Lausted C, Hood L, Fang Q, Wang H, Hu Z. Rapid screening of peptide probes through in situ single-bead sequencing microarray. Anal Chem. 2014 Dec 2;86(23):11854-9. 中科院1区Top期刊

部分授权发明专利
1.用于细胞筛选的微流控芯片及其应用；专利号：ZL202211388875.4
2.实时监测活细胞内ATP动态变化的纳米传感器及其应用；专利号：ZL202211411429.0
3.核酸检测芯片和核酸检测方法；专利号：ZL202211442730.8
4.一种可溶性微针光动力治疗装置；专利号：ZL202111551771.6
5.一种可溶性微针及进药装置；发明人：专利号：ZL202111551742.X
6.一种流式电穿孔装置及系统；专利号：ZL201010242144.X
7.一种单细胞分析用微流控芯片和系统及单细胞分析方法；专利号：ZL201410141283.1
8.一种微流控芯片及其应用；专利号：ZL202110246978.6
9.用于捕获和鉴定循环肿瘤细胞的微流控芯片；专利号：ZL201611053450.2
10.一种检测单克隆抗体的方法及其应用；专利号：ZL202010710574.